ORIGINAL PAPER
The effect of different physical forms of rapeseed
as a fat supplement on the activity of some enzymes
in the duodenal chyme of dairy cows
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1
Shahrekord University, Animal Science Department, Agricultural College, P.O. Box 115, Shahrekord, Iran
2
Aarhus University, Department of Animal Science, Foulum, DK-8830 Tjele, Denmark
Publication date: 2014-11-27
Corresponding author
A. Moharrery
Shahrekord University, Animal Science Department, Agricultural College, P.O. Box 115, Shahrekord, Iran
J. Anim. Feed Sci. 2014;23(4):293-298
KEYWORDS
ABSTRACT
Studies on nutritional regulation of digestive enzymes in ruminants
are scarce. Fat supplementation of diets for dairy cows changes the supply
of nutrients for absorption and transport. The aim of this experiment was
to study the effect of the physical form of rapeseed (Brassica napus) fat on
the duodenal enzyme profile in dairy cows. Four mid-lactation Danish Holstein
dairy cows were submitted to an experiment with a 4 × 4 Latin square design
and fed four rations containing 50% forage and 50% concentrate on a dry matter
basis. The four rations were: a control ration with rapeseed meal (low fat)
and three fat-supplemented rations with either rapeseed cake (RSC), whole
cracked rape seed (WCR), or rapeseed oil (RSO). The correlation coefficients
among duodenal enzyme activities and the relationship between α-amylase
and protease activities were examined. Diurnal samples were taken from
the duodenum, frozen immediately and stored frozen (–20°C) before analyses.
Additional fat increased the soluble protein content in duodenal chyme
(P < 0.05). Neither α-amylase nor the protease or soluble sugar concentrations
were affected by the treatments. Lipase activity increased for RSO compared
with the control. The relationship between α-amylase and protease activities
showed a parabolic behaviour (r = 0.77; P = 0.061). In conclusion, inclusion
of rapeseed oil in the ration of dairy cows under the experimental conditions
increased the soluble protein concentration and lipase activity in duodenal
chyme.
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