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SHORT COMMUNICATION
The profile of gene expression during bovine
adipogenesis: Cloning and expression of type XII
collagen isoforms
1
Cellular Biology Laboratory, Tohoku University,
Sendai, 981-8555, Japan
2
Department of Advanced Medicine and Development, BML Inc.,
Saitama, 350-1101, Japan
3
Institute of Rural Sciences, University of Walesm
Aberystwyth, Ceredigion, SY23 3AL, UK
Publication date: 2004-08-30
J. Anim. Feed Sci. 2004;13(Suppl. 1):369-372
KEYWORDS
ABSTRACT
To isolate candidate genes involved in bovine adipocyte differentiation, we have constructed
a subtraction library from a clonal bovine intramuscular preadipocyte (BIP) cell line using PCRsubtraction. We have isolated a set of subtracted cDNA fragments whose respective mRNA levels
are up regulated during adipogenesis. Two cDNA clones were determined to be type XII collagen
alpha-1. From the expression analysis of type XII collagen, the XIIA-2 isoform was mainly
expressed in differentiated BIP cells and adipose tissues. These results suggest that type XII collagen
may be associated with adipocyte differentiation and adipose formation in cattle and is a potentially
useful marker for adipogenesis.
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| ISSN: | 1230-1388 |
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