ORIGINAL PAPER
The use of developed chromatographic techniques
to determine vitamin E and conjugated linoleic acid
isomers in milk of cows
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1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences,
05-110 Jabłonna, Poland
2
Danish Institute of Agricultural Sciences, Department of Animal Health,
Welfare and Nutrition, Research Centre Foulum,
DK-8830 Tjele, Denmark
Publication date: 2007-01-26
Corresponding author
M. Czauderna
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences,
05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2007;16(Suppl. 1):90-96
KEYWORDS
ABSTRACT
The effect of vitamin E in feed enriched in rape seed oil or sunflower oil on the concentration of
vitamin E in the cow milk was studied by chromatographic assay. The experiment was conducted
on forty Holstein-Friesian cows. Addition of sunflower oil to the feed resulted in lowering the
concentration in the milk compared with the concentration of vitamin E in the milk of cows fed the
ration with rape seed oil. Described GLC procedure provided the new accurate analytical tool for
quantification of CLA isomers and other fatty acids in milk and blood plasma of cows.