Xylanolytic activity of the rumen protozoan
 Diploplastron affine

Cell free extract prepared from ciliates Diploplastron affine free from bacteria degraded xylan releasing reducing sugars at the rate of 117.8 ± 3.14 μM/mg protein/h. Two xylan degrading enzymes have been identified in protozoal cell free extract by a zymogram technique it was also found that one of the identified enzymes degraded xylan to xylooligosaccharides i.e. was of endoxylanase character. Screening of the Diploplastron affine cDNA library resulted in the identification of a xylanase encoding gene consisting of 1670 bp.

CITATIONS (2):

Rumen Protozoa Play a Significant Role in Fungal Predation and Plant Carbohydrate Breakdown
Cate Williams, Benjamin Thomas, Neil McEwan, Stevens Rees, Christopher Creevey, Sharon Huws
Frontiers in Microbiology

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Shifts in xylanases and the microbial community associated with xylan biodegradation during treatment with rumen fluid
Shuhei Takizawa, Ryoki Asano, Yasuhiro Fukuda, Yasunori Baba, Chika Tada, Yutaka Nakai
Microbial Biotechnology

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1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.