Current issue
Online first
Archive
About the Journal
Editorial Office
Editorial Board
Copy right and self-archiving policy
Peer review process
Instructions for Reviewers
Printed version subscription
Abstracting and indexing
Contact
Instructions for Authors
Policies
General information
Open Access, Licensing terms, Commercial use and Copyright terms policies
Self-archiving policy and Archive policies
Article Correction and Withdrawal policy
Manuscript Submission policy
Authorship policy
Conflict of Interest policy
Language considerations policy
Plagiarism and Duplicate publications policy
Ethics policy
Review process policy
Acceptance of manuscripts policy
Online First Articles and Special Issues policies
Generative artificial intelligence (AI) policy
Advertising policy
Article publication charges
Policies
General information
Open Access, Licensing terms, Commercial use and Copyright terms policies
Self-archiving policy and Archive policies
Article Correction and Withdrawal policy
Manuscript Submission policy
Authorship policy
Conflict of Interest policy
Language considerations policy
Plagiarism and Duplicate publications policy
Ethics policy
Review process policy
Acceptance of manuscripts policy
Online First Articles and Special Issues policies
Generative artificial intelligence (AI) policy
Advertising policy
ORIGINAL PAPER
Effect of dietary wheat gluten levels on intestinal mucin flow and composition in young pigs
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
Publication date: 2024-08-02
Corresponding author
E. Święch
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2024;33(4):517-524
KEYWORDS
TOPICS
ABSTRACT
The aim of the study was to investigate the effect of dietary levels
of wheat gluten (WG) protein on the ileal flow of crude mucin and sugars, as well
as sugar composition of crude mucin preparations in young pigs. Six cannulated
young pigs were used in 3 periods × 3 diets in the Latin square design. The three
isoenergetic diets containing low, medium or high WG protein level (LWG, MWG
and HWG, respectively) were formulated. LWG diet was treated as a control.
The three dietary WG levels were obtained by supplementing 26.7, 52.9 and
79.0 g WG/kg to the respective diets. Ileal digesta was collected during the last
3 days of each 7-day period. Xylose content in ileal digesta was higher in LWG
pigs compared to two other pigs groups. Galactose content was higher in MWG
pigs compared to LWG and was lower compared to HWG pigs. Xylose content
decreased linearly and galactose content increased linearly with increasing
dietary WG protein levels. The HWG diet increased ileal flow of crude mucin and
galactose compared to the LWG diet. The flow of crude mucin and galactose
increased linearly with increasing dietary WG protein levels. The content of
galactose in crude mucin preparations was higher in MWG pigs than in LWG
pigs and was lower than in the HWG pigs. Dietary WG protein levels had
a linear effect on the content of xylose and galactose in crude mucin preparations.
In conclusion, WG protein positively affected ileal flow of mucin and its sugar
composition.
ACKNOWLEDGEMENTS
This work was supported by The National Science Centre, Poland (grant No. N311 316736).
CONFLICT OF INTEREST
The Author declares that there is no conflict of interest.
REFERENCES (31)
1.
AOAC International, 2011. Official Methods of Analysis of AOAC International. 18th Ed. Galthersburg, MA (USA).
2.
Bansil R., Turner B.S., 2006. Mucin structure, aggregation, physiological functions and biochemical applications. Curr. Opin. Colloid and Interface Sci., 11, 164–170, https://doi.org./10.1016/j.coc....
3.
Blasco M., Fondevila M., Guada J.A., 2005. Inclusion of wheat gluten as a protein source in diets for weaned pigs. Anim. Res. 54, 297–306, https://doi.org./10.1051/animr....
4.
Izhar M., Nuchamowitz Y., Mirelman D., 1982. Adhere of Shigella flexneri to guinea pig intestinal cells is mediated by mucosal adhesion. Infect. Immun. 35, 1110–1118, https://doi.org./10.1128/iai.3....
5.
Kimura F.T., Miller V.L., 1957. Improved determination of chromic oxide in cow feed and feces. J. Agric. Food Chem. 5, 216, https://doi.org./10.1021/JF600....
6.
Lien K.A., McBurney M.I., Beyde B.I., Thomson, A.B.R., Sauer W.C., 1996. Ileal recovery of nutrients and mucin in humans fed total enteral formulas supplemented with soy fiber. Am. J. Clin. Nutr. 63, 584–595, https://doi.org./10.1093/ajcn/....
7.
Lien K.A., Sauer Q.C., Fenton M., 1997. Mucin output in ileal digesta of pigs fed a protein-free diet. Z. Ernährungwiss. 36, 182–190, https://doi.org./10.1007/BF016....
8.
Lien K.A., Sauer W.C., He J.M., 2001. Dietary influences on the secretion into and degradation of mucin in the digestive tract of monogastric animals and humans. J. Anim. Feed Sci. 10, 223–245, https://doi.org./10.22358/jafs....
9.
Miner-Williams W.M., Moughan P. J., Fuller M.F., 2009a. Endogenous components of digesta protein from the terminal ileum of pigs fed a casein-based diet. J. Agric. Food Chem. 57, 2072–2078, https://doi.org./10.1021/jf802....
10.
Miner-Williams W.M., Moughan P.J., Fuller M.F., 2009b. Methods for mucin analysis. A comparative study. J. Agric. Food Chem. 57, 6029–6035, https://doi.org./10.1021/jf901....
11.
Miner-Williams W.M., Moughan P.J., Fuller M.F., 2013. Analysis of ethanol precipitate from ileal digesta. Sci. Rep. 3, 3145, https://doi.org./10.1038/srep0....
12.
Montagne L., Piel M.S., Lallès J.-P., 2004. Effect of diet on mucin kinetics and composition: nutrition and health implications. Nutr. Rev. 62, 105–114, https://doi.org./10.1111/j.175....
13.
Montagne L., Toullec R., Formal M., Lallès J.-P., 2000a. Influence of dietary protein level and origin on the flow of mucin along the small intestine of the preruminant calf. J. Dairy Sci. 83, 2820–2828, https://doi.org./10.3168/jds.S....
14.
Montagne L., Toullec R., Lallès J.-P., 2000b. Calf intestinal mucin: isolation, partial characterization, and measurement in ileal digesta with an enzyme-linked immunosorbent assay. J. Dairy Sci. 83, 507–517, https://doi.org./10.3168/jds.S....
15.
Montoya C.A., Leterme P., Romé V., Beebe S., Claustre J., Lallès J.-P., 2010. Phaseolin from Phaseolus vulgaris bean modulates gut mucin flow and gene expression in rats. Br. J. Nutr. 104, 1740–1747, https://doi.org./10.1017/S0007....
16.
NRC (National Research Council), 1998. Nutrient Requirements of Swine. 10th Ed. The National Academy Press. Washington, DC (USA).
17.
Opapeju F.O., Rademacher M., Blank G., Nyachoti C.M., 2008. Effect of low-protein amino acid-supplemented diet on the growth performance, gut morphology, organ weights and digesta characteristics of weaned pigs. Animal 2, 1457–1464, https://doi.org./10.1017/S1751....
18.
Piel C., Montagne L., Saldago P., Lallès, J.-P., 2004. Estimation of ileal output of gastro-intestinal glycoprotein in weaned piglets using three different methods. Reprod. Nutr. Dev. 44, 419–435, https://doi.org./10.1051/rnd:2....
19.
Piel C., Montagne L., Sève B., Lallès, J.-P., 2005. Increasing digesta viscosity using carboxymethylcellulose in weaned piglets stimulates ileal goblet cell numbers and maturation. J. Nutr. 135, 86–91, https://doi.org./10.1093/jn/13....
20.
Piel C., Montagne L., Sève B., Lallès J.-P., 2007. Dietary fibre and indigestible protein increase ileal glycoprotein output without impacting colonic crypt goblet cells in weaned piglets. Livest. Sci. 108, 106–108, https://doi.org./10.1016/j.liv....
21.
Rathod A.K., Dhok A.P., Faecal biomarkers of gastrointestinal functionality in animals. J. Anim. Feed Sci. 33, 149–158, https://doi.org./10.22358/jafs....
22.
Reeds P.J., Burrin D.G., 2001. Glutamine and the bowel. J. Nutr. 131, 2505S–2508S, https://doi.org./10.1093/jn/13....
23.
Święch E., 2015. Utilization of threonine in young pigs fed diets differing in standardized ileal digestible threonine and wheat gluten used as a source of non-essential amino acids. J. Anim. Feed Sci. 24, 323–331, https://doi.org./10.22358/jafs....
24.
Święch E., Boryczka M., Taciak M., Tuśnio A., Buraczewska L., 2010. Goblet cells in the small intestine in young pigs fed low-threonine diet supplemented with threnine or with different sources of non essential amino acids. In. G.M. Crovetto (Ed.), Energy and Protein Metabolism and Nutrition. Proceedings of the 3rd EAAP International Symposium on Energy and Protein Metabolism and Nutrition. Parma (Italy). EAAP publication No. 127. Wageningen Academic Publisher, pp. 207–208.
25.
Święch E., Tuśnio A., Taciak M., Barszcz M., 2022. Modulation of mucin secretion in the gut of young pigs by dietary threonine and non-essential amino acid levels. Animals 12, 270, https://doi.org./10.3390/ani12....
26.
Trompette A., Claustre J., Caillon F., Jourdan G., Chayvialle J.A., Plaisancié G., 2003. Milk bioactive peptides and β-casomorphins induce mucus release in rat jejunum. J. Nutr. 133, 3499–3503, https://doi.org./10.1093/jn/13....
27.
Tsirtsikos P., Fegeros K., Balaskas C., Kominakis A., Mountzouris K.C., 2012a. Dietary probiotic inclusion level modulates intestinal mucin composition and mucosal morphology in broilers. Poult. Sci. 91, 1860–1868, https://doi.org./10.3382/ps.20....
28.
Tsirtsikos P., Fegeros K., Kominakis A., Balaskas C., Mountzouris K.C., 2012b. Modulation of intestinal mucin composition and mucosal morphology of dietary phytogenic inclusion level in broilers. Animal 6, 1049–1057, https://doi.org./10.1017/S1751....
29.
van Leeuwen P., van Kleef D., van Kempen G.J.M., Huisman J., Verstegen M.W.A., 1991. The post valve T-caecum cannulation technique in pigs applicated to determine the digestibility of amino acid in maize, groundnut and sunflower meal. Anim. Physiol. Anim. Nutr. 65, 183–193, https://doi.org./10.1111/j.143....
30.
Wu G., Bazar F.W., Johnson G.A., Knabe D.A., Burghardt R.C., Spencer T.E., Liu X.L., Wang, J.J., 2011. Triennial growth symposium: Important roles for L-glutamine in swine nutrition and production. J. Anim. Sci. 2011, 89, 2017-2030, https://doi.org./10.2527/jas.2....
31.
Xia J., Fan H., Yang J., Song T., Pang L., Deng H., Ren Z., Deng J., 2022. Research progress on diarrhea and its mechanism in weaned piglets fed a high-protein diet. J. Anim. Physiol. Anim. Nutr. 106, 1277–1287, https://doi.org./10.1111/jpn.1....
Share
RELATED ARTICLE
| ISSN: | 1230-1388 |
Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.
© 2006-2025 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
