Misuse of antibiotics in feed seriously affects pig intestinal health. Alternative feed additives are vital for maintaining pig health and productivity. This study investigated the effects of glucose oxidase (GOD) and probiotics on pig growth and the morphology of microfold cells in the small intestine. A total of 160 40-day-old (half males and half females) weaning piglets from the Duroc × Large White × Yorkshire (DLY) crossbreed was randomly assigned into 4 treatment groups with 4 replicates of 10 pigs per group. The dietary treatments included: (1) control (commercial basal diet without additive), (2) basal diet with antibiotics (50% gentamicin, 0.19 kg/t, 10% clomiphene, 0.42 kg/t, 10% bacitracin zinc, 0.44 kg/t), (3) basal diet with GOD (0.5 kg/t, 3000 U/g), and (4) basal diet with probiotics (Lactobacillus 3.0 × 10⁹ CFU/g, Bacillus subtilis 1.4 × 10¹⁰ CFU/g, B. licheniformis 1.3 ×10¹⁰ CFU/g) and GOD. The trial included a 7-day preliminary period and a 35-day formal testing period. Results showed that the combination of probiotics and GOD significantly increased average daily gain (ADG) and reduced feed-to-gain ratio (F/G) of pigs (P < 0.05). The intestinal histology results indicated that antibiotics in feed severely damaged the morphology of intestinal microvilli and microfold cells, disrupting the intestinal microflora in nursery pigs. While GOD altered the morphology of microfold cells in the small intestine, enhancing phagocytosis, it also caused some damage to the intestinal mucosa. However, the combined application of probiotics and GOD helped repair the intestinal mucosa damage caused by GOD. In summary, the combination of probiotics and GOD as feed additives can serve as an effective alternative to antibiotics, improving both the growth and immune performance of nursery pigs